Novel insights into the histology of jejunoileal atresia and its therapeutic implications
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Topic overview
Abstract
Aim
To study the histology of resected specimens of jejunoileal atresia (JIA) and to explore its probable therapeutic implications.
Methods
Biopsies were taken from the resected specimens of 32 patients of JIA. Sections were taken at the atretic ends and successively at every 5 cm in resected proximal bowel. They were stained for light microscopy and immunohistochemistry for CD117 (Interstitial cell of Cajal), α smooth muscle antibody (SMA), neuron filament protein (NFP) and calretinin. Histological findings on light microscopy and immune reactivity intensity for NFP, calretinin and α-SMA were recorded. Time taken to achieve full enteral nutrition (FEN; defined as attainment of oral feeds at 100 ml/kg/day without any intravenous fluid supplementation) was recorded and correlated with CD117 count by non-parametric Spearman rank correlation coefficient.
Results
Light microscopy failed to detect any abnormality in majority (n = 27, 84%) of JIA specimens. Mucosal ulceration (7.8%), submucosal inflammatory cells (8%), focal muscle thinning (2.5%), decreased ganglion cells and nerve fibers (3.8%) were noted on light microscopy. However all of them had normal α SMA, calretinin and NFP immunoreactivity. Mean ICC counts at proximal and distal atretic segment were 6.56 ± 3.79 and 8.37 ± 3.21 per HPF respectively. Serial sections in proximal dilated segment did not reveal any definite or well demarcated increase in ICC counts. CD117 counts at the proximal cut end of atresia was less than 6 per high power field (hpf) in 15 patients (46.8%) while at the site of anastomosis a count of more than 6/hpf was observed in 73% patients. There was no direct correlation of ICC counts with attainment of FEN.
Conclusion
Absence of major morphological abnormalities in the proximal dilated bowel contradicts the findings of earlier studies on histology of JIA in smaller cohorts. Our observations reiterate that bowel plication rather than massive bowel resection should be the preferred management in babies with JIA.
Level of evidence
Prognosis study (Level II).
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