Primary exploring the value of metagenomic next‐generation sequencing in detecting pathogenic bacteria of cholangitis with biliary atresia after Kasai operation

Study compares metagenomic next-generation sequencing (mNGS) to traditional blood culture for detecting cholangitis pathogens in 25 biliary atresia patients post-Kasai operation. mNGS demonstrated superior detection rate (73.3% vs 23.3%), faster turnaround time (16 vs 120 hours), and identified broader bacterial spectrum, supporting its use for targeted antibiotic therapy in this high-risk population.