Image Processing and Analysis of Mucosal Calretinin Staining to Define the Transition Zone in Hirschsprung Disease: A Pilot Study

Pilot study evaluating image processing analysis of calretinin-stained mucosal biopsies to objectively define the transition zone in Hirschsprung disease. While staining intensity gradually increased proximally in 77% of cases, substantial circumferential variation limits precise boundary definition, though the technique shows promise as a quantitative research tool.