Increasing Trend of Calretinin-Positive Mucosal Innervation from Aganglionic Zone toward Transition Zone in Hirschsprung's Disease

Study evaluating calretinin-positive mucosal nerve patterns in Hirschsprung's disease diagnosis found that an increasing gradient of calretinin staining from aganglionic to transition zones was universally present in pull-through specimens. Traditional diagnostic markers showed limitations in very short-segment disease, suggesting image processing analysis of calretinin trends may improve diagnostic accuracy.