A comparison of small bowel and fecal microbiota in children with short bowel syndrome

Space: StayCurrentMD Author: Hannah G. Piper, Laura A. Coughlin, Van Nguyen, Nandini Channabasappa, Andrew Y. Koh Published:

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Hannah G. Piper, Laura A. Coughlin, Van Nguyen, Nandini Channabasappa, Andrew Y. Koh

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Abstract

Background

Babies with short bowel syndrome (SBS) have small intestinal microbial disturbances that impact gut function. Characterizing the small bowel microbiota is challenging, and the utility of sampling stool is unclear. This study compares the microbiota from fecal samples and the small bowel.

Methods

Stool samples were collected (2016–2017) from infants with SBS and colon in continuity (COLON) or SBS with small bowel ostomy (sbSTOMA). The abundance and quantity of major bacterial genera was compared between groups and to healthy controls using 16S rRNA sequencing and qPCR. Kruskall-Wallis test was used for analysis with P values

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