A Comparison between Placental and Amniotic Mesenchymal Stem Cells in Transamniotic Stem Cell Therapy for Experimental Gastroschisis
Author / Expert
Alexander V. Chalphin, Sarah A. Tracy, Stefanie P. Lazow, Ina Kycia, David Zurakowski, Dario O. Fauza
Topic overview
Abstract
Purpose
We compared placental and amniotic fluid-derived mesenchymal stem cells (pMSCs and afMSCs, respectively) in transamniotic stem cell therapy for experimental gastroschisis.
Methods
Gastroschisis was surgically created in 126 rat fetuses at gestational day 18 (term = 22 days), immediately followed by volume-matched intraamniotic injections of suspensions of afMSCs (n = 32), pMSCs (n = 33), or normal saline (NS) (n = 33). Untreated fetuses served as controls (n = 28). Blinded observers performed computerized measurements of total and segmental (serosa, muscularis, and mucosa) intestinal wall thickness on the herniated bowel at term. Statistical analysis included ANOVA, the Wald test, and Levene's test (p
Comments