First steps in the development of a liquid biopsy in situ hybridization protocol to determine circular RNA biomarkers in rat biofluids

Researchers adapted BaseScope in situ hybridization to detect circular RNAs in rat serum and amniotic fluid, demonstrating feasibility for prenatal CDH biomarker profiling. The protocol successfully identified dysregulated circRNAs in nitrofen-induced CDH model biofluids using fluorescent detection, establishing proof-of-concept for liquid biopsy approaches in congenital diaphragmatic hernia diagnostics.