the reversal group very closely mirrors the composition of the child group, both in its baseline composition and in its response to surgery, suggesting that the reversal in phenotype we saw may be due to changes in the microbiome.
the reversal group very closely mirrors the composition of the child group, both in its baseline composition and in its response to surgery, suggesting that the reversal in phenotype we saw may be due to changes in the microbiome.
the reversal group very closely mirrors the composition of the child group, both in its baseline composition and in its response to surgery, suggesting that the reversal in phenotype we saw may be due to changes in the microbiome.
with diet reversal experiments, we're able to show that preoperative diet manipulation reduces tumor formation by suggesting that there may be a clinically relevant role for diet rehabilitation and colorectal cancer treatment.
Best of the Best Gen Surg - Development of postoperative local tumors and distant metastases in diet, genetics and microbiome-dependent in a mouse model of colorectal cancer recurrence - Dr. Morgan
▶Ep 127 · 0:45
clinicalTraditional paradigms suggest recurrences are due to either incomplete resection or occult metastases left behind at the time of surgery.↗
▶Ep 127 · 0:45
epidemiologicalColorectal cancer is a leading cause of cancer deaths, particularly in cases of recurrence.↗
▶Ep 127 · 0:45
epidemiologicalColorectal cancer is a leading cause of cancer deaths, particularly in cases of recurrence.↗
▶Ep 127 · 0:45
host_summaryTraditional paradigms suggest recurrences are due to either incomplete resection or occult metastases left behind at the time of surgery.↗
▶Ep 127 · 0:58
clinicalExfoliated cancer cells in the lumen of the bowel may be a source of recurrence.↗
▶Ep 127 · 0:58
host_summaryExfoliated cancer cells in the lumen of the bowel may be a source of recurrence.↗
▶Ep 127 · 1:04
host_summaryIn the 1980s, UMLB showed that exfoliated cancer cells are present and viable at the time of surgery.↗
▶Ep 127 · 1:04
clinicalIn the 1980s, UMLB showed that exfoliated cancer cells are present and viable at the time of surgery.↗
▶Ep 127 · 1:09
host_summaryExperimental cells could cross a watertight anastomosis in a mouse model.↗
▶Ep 127 · 1:09
clinicalExperimental cells could cross a watertight anastomosis in a mouse model.↗
▶Ep 127 · 1:14
host_summaryRectal washout decreases rates of local recurrence after rectal cancer resection.↗
▶Ep 127 · 1:14
clinicalRectal washout decreases rates of local recurrence after rectal cancer resection.↗
▶Ep 127 · 1:39
clinicalWhen the primary colorectal tumor is resected, exfoliated cells remain behind within the lumen of the bowel and interact with the anastomotic microenvironment.↗
▶Ep 127 · 1:39
clinicalWhen the primary colorectal tumor is resected, exfoliated cells remain behind within the lumen of the bowel and interact with the anastomotic microenvironment.↗
▶Ep 127 · 1:48
clinicalMultiple factors can influence the anastomotic environment, including diet, the microbiome, and the tumor genetic background.↗
▶Ep 127 · 1:48
clinicalMultiple factors can influence the anastomotic environment, including diet, the microbiome, and the tumor genetic background.↗
▶Ep 127 · 1:55
quoteWe propose that the interaction of these factors in the healing anastomosis promotes the migration of these cells to cause both local and distant recurrences.↗
▶Ep 127 · 1:55
quoteWe propose that the interaction of these factors in the healing anastomosis promotes the migration of these cells to cause both local and distant recurrences.↗
▶Ep 127 · 2:04
clinicalA high-fat diet and its impact on the microbiome would alter the anastomotic environment and change the metastatic potential of cells.↗
▶Ep 127 · 2:04
quoteour hypothesis is that a high fat diet and its impact on the microbiome would alter the anastomotic environment and change the metastatic potential of cells.↗
▶Ep 127 · 2:04
quoteour hypothesis is that a high fat diet and its impact on the microbiome would alter the anastomotic environment and change the metastatic potential of cells.↗
▶Ep 127 · 2:04
clinicalA high-fat diet and its impact on the microbiome would alter the anastomotic environment and change the metastatic potential of cells.↗
▶Ep 127 · 2:15
host_summaryAKPT and KPN organoid cell lines demonstrate a wide range of metastatic potential in animal models.↗
▶Ep 127 · 2:15
clinicalAKPT and KPN organoid cell lines demonstrate a wide range of metastatic potential in animal models.↗
▶Ep 127 · 3:35
clinicalAKPT mice showed significantly higher rate of tumor formation with a high-fat diet compared to mice given a chow diet by 56 days.↗
▶Ep 127 · 3:35
clinicalAKPT mice showed significantly higher rate of tumor formation with a high-fat diet compared to mice given a chow diet by 56 days.↗
▶Ep 127 · 3:46
clinicalKPN mice showed no significant difference in tumor formation based on diet, with an opposite trend towards higher rates in the chow diet.↗
▶Ep 127 · 3:46
clinicalKPN mice showed no significant difference in tumor formation based on diet, with an opposite trend towards higher rates in the chow diet.↗
▶Ep 127 · 4:08
clinicalAKPT mice given a high-fat diet had significantly decreased survival compared to those on a chow diet.↗
▶Ep 127 · 4:08
clinicalAKPT mice given a high-fat diet had significantly decreased survival compared to those on a chow diet.↗
▶Ep 127 · 4:14
clinicalKPN mice did not show any similar survival trend, suggesting that response to diet was at least in part based on the organoid genetic background.↗
▶Ep 127 · 4:14
clinicalKPN mice did not show any similar survival trend, suggesting that response to diet was at least in part based on the organoid genetic background.↗
▶Ep 127 · 4:57
quoteWhen isolated in a germ-free mouse model, the high fat diet associated microbiome significantly increased the rate of post-operative tumor development.↗
▶Ep 127 · 4:57
clinicalIn germ-free mice, the high-fat diet-associated microbiome significantly increased the rate of postoperative tumor development (40% vs 10-12% in control and chow FMT groups).↗
▶Ep 127 · 4:57
clinicalIn germ-free mice, the high-fat diet-associated microbiome significantly increased the rate of postoperative tumor development (40% vs 10-12% in control and chow FMT groups).↗
▶Ep 127 · 4:57
quoteWhen isolated in a germ-free mouse model, the high fat diet associated microbiome significantly increased the rate of post-operative tumor development.↗
▶Ep 127 · 5:50
clinicalTwo-week diet reversal period was insufficient; mice in the reversal group continued to have poor survival and high tumor rates similar to the high-fat diet group.↗
▶Ep 127 · 5:50
clinicalTwo-week diet reversal period was insufficient; mice in the reversal group continued to have poor survival and high tumor rates similar to the high-fat diet group.↗
▶Ep 127 · 6:01
clinicalSix-week diet reversal resulted in significantly increased survival and tumor rate similar to chow-fed mice, suggesting the response to diet and its reversal was time dependent.↗
▶Ep 127 · 6:01
clinicalSix-week diet reversal resulted in significantly increased survival and tumor rate similar to chow-fed mice, suggesting the response to diet and its reversal was time dependent.↗
▶Ep 127 · 6:30
clinicalHigh-fat diet mice showed derangement of microbiome with overabundance of Alobaum and bloom of pathogenic bacteria such as Proteus, E. coli, and Shigella on postoperative day 7.↗
▶Ep 127 · 6:30
clinicalHigh-fat diet mice showed derangement of microbiome with overabundance of Alobaum and bloom of pathogenic bacteria such as Proteus, E. coli, and Shigella on postoperative day 7.↗
▶Ep 127 · 6:45
clinicalThe diet reversal group microbiome composition closely mirrored the chow group both in baseline composition and in response to surgery.↗
▶Ep 127 · 6:45
quotethe reversal group very closely mirrors the composition of the child group, both in its baseline composition and in its response to surgery, suggesting that the reversal in phenotype we saw may be due to changes in the microbiome.↗
▶Ep 127 · 6:45
quotethe reversal group very closely mirrors the composition of the child group, both in its baseline composition and in its response to surgery, suggesting that the reversal in phenotype we saw may be due to changes in the microbiome.↗
▶Ep 127 · 6:45
clinicalThe diet reversal group microbiome composition closely mirrored the chow group both in baseline composition and in response to surgery.↗
▶Ep 127 · 7:23
opinionPreoperative diet manipulation reduces tumor formation, suggesting a clinically relevant role for diet rehabilitation in colorectal cancer treatment.↗
▶Ep 127 · 7:23
quotewith diet reversal experiments, we're able to show that preoperative diet manipulation reduces tumor formation by suggesting that there may be a clinically relevant role for diet rehabilitation and colorectal cancer treatment.↗
▶Ep 127 · 7:23
quotewith diet reversal experiments, we're able to show that preoperative diet manipulation reduces tumor formation by suggesting that there may be a clinically relevant role for diet rehabilitation and colorectal cancer treatment.↗
▶Ep 127 · 7:23
opinionPreoperative diet manipulation reduces tumor formation, suggesting a clinically relevant role for diet rehabilitation in colorectal cancer treatment.↗
▶Ep 127 · 9:23
clinicalCombined mechanical and antibiotic bowel preparation preoperatively decreases rates of colorectal cancer recurrence.↗
▶Ep 127 · 9:23
host_summaryCombined mechanical and antibiotic bowel preparation preoperatively decreases rates of colorectal cancer recurrence.↗
▶Ep 127 · 11:05
clinicalPreliminary in vitro data shows AKPT cells have more proliferative response to secondary bile acids (like deoxycholic acid) compared to KPN cells.↗
▶Ep 127 · 11:05
clinicalSecondary bile acids and deoxycholic acid metabolites are exclusively bacterially produced.↗
▶Ep 127 · 11:05
clinicalSecondary bile acids and deoxycholic acid metabolites are exclusively bacterially produced.↗
▶Ep 127 · 11:05
clinicalPreliminary in vitro data shows AKPT cells have more proliferative response to secondary bile acids (like deoxycholic acid) compared to KPN cells.↗
Best of the Best Gen Surg - Development of postoperative local tumors and distant metastases in diet, genetics and microbiome-dependent in a mouse model of colorectal cancer recurrence - Dr. Morgan
▶Ep 1 · 0:45
epidemiologicalColorectal cancer is a leading cause of cancer deaths, particularly in cases of recurrence.↗
▶Ep 1 · 0:45
host_summaryTraditional paradigms suggest recurrences are due to either incomplete resection or occult metastases left behind at the time of surgery.↗
▶Ep 1 · 0:58
host_summaryExfoliated cancer cells in the lumen of the bowel may be a source of recurrence.↗
▶Ep 1 · 1:04
host_summaryIn the 1980s, UMLB showed that exfoliated cancer cells are present and viable at the time of surgery.↗
▶Ep 1 · 1:09
host_summaryExperimental cells could cross a watertight anastomosis in a mouse model.↗
▶Ep 1 · 1:14
host_summaryRectal washout decreases rates of local recurrence after rectal cancer resection.↗
▶Ep 1 · 1:39
clinicalWhen the primary colorectal tumor is resected, exfoliated cells remain behind within the lumen of the bowel and interact with the anastomotic microenvironment.↗
▶Ep 1 · 1:48
clinicalMultiple factors can influence the anastomotic environment, including diet, the microbiome, and the tumor genetic background.↗
▶Ep 1 · 1:55
quoteWe propose that the interaction of these factors in the healing anastomosis promotes the migration of these cells to cause both local and distant recurrences.↗
▶Ep 1 · 2:04
quoteour hypothesis is that a high fat diet and its impact on the microbiome would alter the anastomotic environment and change the metastatic potential of cells.↗
▶Ep 1 · 2:04
clinicalA high-fat diet and its impact on the microbiome would alter the anastomotic environment and change the metastatic potential of cells.↗
▶Ep 1 · 2:15
host_summaryAKPT and KPN organoid cell lines demonstrate a wide range of metastatic potential in animal models.↗
▶Ep 1 · 3:35
clinicalAKPT mice showed significantly higher rate of tumor formation with a high-fat diet compared to mice given a chow diet by 56 days.↗
▶Ep 1 · 3:46
clinicalKPN mice showed no significant difference in tumor formation based on diet, with an opposite trend towards higher rates in the chow diet.↗
▶Ep 1 · 4:08
clinicalAKPT mice given a high-fat diet had significantly decreased survival compared to those on a chow diet.↗
▶Ep 1 · 4:14
clinicalKPN mice did not show any similar survival trend, suggesting that response to diet was at least in part based on the organoid genetic background.↗
▶Ep 1 · 4:57
clinicalIn germ-free mice, the high-fat diet-associated microbiome significantly increased the rate of postoperative tumor development (40% vs 10-12% in control and chow FMT groups).↗
▶Ep 1 · 4:57
quoteWhen isolated in a germ-free mouse model, the high fat diet associated microbiome significantly increased the rate of post-operative tumor development.↗
▶Ep 1 · 5:50
clinicalTwo-week diet reversal period was insufficient; mice in the reversal group continued to have poor survival and high tumor rates similar to the high-fat diet group.↗
▶Ep 1 · 6:01
clinicalSix-week diet reversal resulted in significantly increased survival and tumor rate similar to chow-fed mice, suggesting the response to diet and its reversal was time dependent.↗
▶Ep 1 · 6:30
clinicalHigh-fat diet mice showed derangement of microbiome with overabundance of Alobaum and bloom of pathogenic bacteria such as Proteus, E. coli, and Shigella on postoperative day 7.↗
▶Ep 1 · 6:45
clinicalThe diet reversal group microbiome composition closely mirrored the chow group both in baseline composition and in response to surgery.↗
▶Ep 1 · 6:45
quotethe reversal group very closely mirrors the composition of the child group, both in its baseline composition and in its response to surgery, suggesting that the reversal in phenotype we saw may be due to changes in the microbiome.↗
▶Ep 1 · 7:23
quotewith diet reversal experiments, we're able to show that preoperative diet manipulation reduces tumor formation by suggesting that there may be a clinically relevant role for diet rehabilitation and colorectal cancer treatment.↗
▶Ep 1 · 7:23
opinionPreoperative diet manipulation reduces tumor formation, suggesting a clinically relevant role for diet rehabilitation in colorectal cancer treatment.↗
▶Ep 1 · 9:23
host_summaryCombined mechanical and antibiotic bowel preparation preoperatively decreases rates of colorectal cancer recurrence.↗
▶Ep 1 · 11:05
clinicalPreliminary in vitro data shows AKPT cells have more proliferative response to secondary bile acids (like deoxycholic acid) compared to KPN cells.↗
▶Ep 1 · 11:05
clinicalSecondary bile acids and deoxycholic acid metabolites are exclusively bacterially produced.↗
