15 statements
· 1 topic
· summaries given as host listed separately
Featured statements
▶Ep 20 · 1:00
necrotizing enterocolitis, or neck is a devastating gastrointestinal disease in neonates. It starts with severe intestinal inflammation, which can lead to intestinal necrosis and perforation and ultimately multi-system organ failure.
Fluorescently tagged microRNA-148a was present in the cytosol of intestinal epithelial cells after LNP administration via enema in healthy neonatal mice, demonstrating successful delivery.
Mina Yeganeh, BSc - Best of the Best in Pediatric Surgery 2024
▶Ep 20 · 0:41
clinicalNecrotizing enterocolitis starts with severe intestinal inflammation, which can lead to intestinal necrosis and perforation and ultimately multi-system organ failure.↗
▶Ep 20 · 1:00
quotenecrotizing enterocolitis, or neck is a devastating gastrointestinal disease in neonates. It starts with severe intestinal inflammation, which can lead to intestinal necrosis and perforation and ultimately multi-system organ failure.↗
▶Ep 20 · 1:55
quoteDespite these protective effects, the underlying mechanisms of milk EVs remain unclear, preventing their clinical translation.↗
▶Ep 20 · 2:00
clinicalMicroRNA-148a is the most abundant microRNA in milk extracellular vesicles.↗
▶Ep 20 · 2:45
clinicalTLR-4 is a pattern recognition receptor that is critical for the initiation of inflammatory response.↗
▶Ep 20 · 4:00
clinicalLipid nanoparticles encapsulate nucleic acids and release them inside cells in a pH responsive manner.↗
▶Ep 20 · 4:10
clinicalFluorescently tagged microRNA-148a was present in the cytosol of intestinal epithelial cells after LNP administration via enema in healthy neonatal mice, demonstrating successful delivery.↗
▶Ep 20 · 4:30
clinicalAdministration of microRNA-148a and TLR-4 siRNA antagonist both significantly reduced intestinal injury in experimental NEC compared to untreated NEC mice.↗
▶Ep 20 · 4:45
clinicalNEC results in significant increase in gene expression of pro-inflammatory cytokines TNF-α and IL-6 in the intestine compared to control mice.↗
▶Ep 20 · 5:00
clinicalAdministration of microRNA-148a and TLR-4 antagonists both result in significantly lower inflammation (reduced TNF-α and IL-6 expression) in the intestine during experimental NEC.↗
▶Ep 20 · 5:15
clinicalNF-κB is a transcription factor downstream of TLR-4.↗
▶Ep 20 · 5:20
clinicalLipopolysaccharide activates the Toll-like receptor pathway and causes significant rise in NF-κB expression.↗
▶Ep 20 · 5:28
clinicalMicroRNA-148a and TLR-4 antagonist both significantly lower NF-κB expression at two different concentrations when administered with LPS.↗
▶Ep 20 · 5:54
clinicalIn the experimental NEC model used (P5 to P9), the surge in intestinal injury and inflammation occurs around P6 and increases from P6 to P7.↗
▶Ep 20 · 5:54
quoteit's really hard to distinguish between preventative and therapeutic, uh, interventions with the, uh, experimental model that we have right now for neck.↗
Summaries Mina gave as host
· 4 summaries
Recaps of other experts' statements, not Mina's own clinical position.
Summaries Mina gave as host · Necrotizing Enterocolitis4 summaries
Mina Yeganeh, BSc - Best of the Best in Pediatric Surgery 2024
▶Ep 20 · 1:30
host summaryMina Yeganeh summarizing a resource: Administration of human milk extracellular vesicles protects mice from developing NEC, with significantly lower intestinal injury scores and lower intestinal inflammation compared to untreated NEC.↗
▶Ep 20 · 2:20
host summaryMina Yeganeh summarizing a resource: MicroRNA-148a has shown to reduce the inflammatory response in other disease models.↗
▶Ep 20 · 2:30
host summaryMina Yeganeh summarizing a resource: The anti-inflammatory effect of microRNA-148a is mainly through the inhibition of Toll-like receptor 4 (TLR-4) pathway.↗
▶Ep 20 · 2:55
host summaryMina Yeganeh summarizing a resource: Dr. David Hackam's group has shown that TLR-4 signaling is required for the development of experimental NEC.↗
