The aim of our study was to determine if extracellular vesicles from osteosarcoma can establish a pre-metastatic niche and increase pulmonary metastasis in a mirroring model of osteosarcoma.
The aim of our study was to determine if extracellular vesicles from osteosarcoma can establish a pre-metastatic niche and increase pulmonary metastasis in a mirroring model of osteosarcoma.
The aim of our study was to determine if extracellular vesicles from osteosarcoma can establish a pre-metastatic niche and increase pulmonary metastasis in a mirroring model of osteosarcoma.
The aim of our study was to determine if extracellular vesicles from osteosarcoma can establish a pre-metastatic niche and increase pulmonary metastasis in a mirroring model of osteosarcoma.
Bailey Roberts, MD - Best of the Best in Pediatric Surgery 2024
▶Ep 3 · 1:04
quoteOsteosarcoma is a malignant pediatric tumor of the bone.↗
▶Ep 3 · 1:04
quoteOsteosarcoma is a malignant pediatric tumor of the bone.↗
▶Ep 3 · 1:08
epidemiologicalOsteosarcoma most commonly affects adolescents and young adults↗
▶Ep 3 · 1:08
epidemiologicalOsteosarcoma most commonly affects adolescents and young adults↗
▶Ep 3 · 1:11
epidemiologicalGross metastasis at the time of diagnosis is present about 20% of the time in osteosarcoma↗
▶Ep 3 · 1:11
clinicalThe most common site of metastasis in osteosarcoma is in the lung↗
▶Ep 3 · 1:11
clinicalThe most common site of metastasis in osteosarcoma is in the lung↗
▶Ep 3 · 1:11
epidemiologicalGross metastasis at the time of diagnosis is present about 20% of the time in osteosarcoma↗
▶Ep 3 · 1:19
epidemiologicalOverall survival in osteosarcoma worsens from 80% to about 20% for metastatic disease↗
▶Ep 3 · 1:19
epidemiologicalOverall survival in osteosarcoma worsens from 80% to about 20% for metastatic disease↗
▶Ep 3 · 1:25
clinicalRecurrence is very common in osteosarcoma, and the most common site of recurrence is in the lungs↗
▶Ep 3 · 1:25
clinicalRecurrence is very common in osteosarcoma, and the most common site of recurrence is in the lungs↗
▶Ep 3 · 1:32
quoteExtracellular vesicles or EVs are small nanoparticles released from all cells.↗
▶Ep 3 · 1:32
quoteExtracellular vesicles or EVs are small nanoparticles released from all cells.↗
▶Ep 3 · 1:38
clinicalExtracellular vesicles are carriers of amino acids, proteins, mRNA or other forms of cellular communication↗
▶Ep 3 · 1:38
clinicalExtracellular vesicles are carriers of amino acids, proteins, mRNA or other forms of cellular communication↗
▶Ep 3 · 1:45
clinicalEV content is unique and specific to the cells that released it, and therefore can be traced back to the cell type↗
▶Ep 3 · 1:45
clinicalEV content is unique and specific to the cells that released it, and therefore can be traced back to the cell type↗
▶Ep 3 · 1:52
clinicalEVs are involved in the metastatic cascade and implicated as potential markers for disease diagnosis and staging↗
▶Ep 3 · 1:52
clinicalEVs are involved in the metastatic cascade and implicated as potential markers for disease diagnosis and staging↗
▶Ep 3 · 1:59
clinicalEVs have been shown to change fibroblasts, inhibit T cell activity, and are involved in establishing a pre-metastatic niche↗
▶Ep 3 · 1:59
clinicalEVs have been shown to change fibroblasts, inhibit T cell activity, and are involved in establishing a pre-metastatic niche↗
▶Ep 3 · 2:14
quoteThe aim of our study was to determine if extracellular vesicles from osteosarcoma can establish a pre-metastatic niche and increase pulmonary metastasis in a mirroring model of osteosarcoma.↗
▶Ep 3 · 2:14
quoteThe aim of our study was to determine if extracellular vesicles from osteosarcoma can establish a pre-metastatic niche and increase pulmonary metastasis in a mirroring model of osteosarcoma.↗
▶Ep 3 · 2:31
clinicalK12 is a spontaneous osteosarcoma with low metastatic capability↗
▶Ep 3 · 2:31
clinicalK12 is a spontaneous osteosarcoma with low metastatic capability↗
▶Ep 3 · 2:35
clinicalK7M2 is a highly metastatic osteosarcoma derived from a spontaneous osteosarcoma that went through two reiterations of harvesting spontaneous lung metastases and reimplanting them into the mouse↗
▶Ep 3 · 2:35
clinicalK7M2 is a highly metastatic osteosarcoma derived from a spontaneous osteosarcoma that went through two reiterations of harvesting spontaneous lung metastases and reimplanting them into the mouse↗
▶Ep 3 · 2:57
clinicalThe K7M2 cell line is more aggressive and causes death at a much higher rate than K12↗
▶Ep 3 · 2:57
clinicalThe K7M2 cell line is more aggressive and causes death at a much higher rate than K12↗
▶Ep 3 · 3:59
clinicalPre-education with K7M2 EVs drastically increases the metastatic burden in the lungs of mice with K7M2 tumors↗
▶Ep 3 · 3:59
clinicalPre-education with K7M2 EVs drastically increases the metastatic burden in the lungs of mice with K7M2 tumors↗
▶Ep 3 · 4:18
clinicalPre-education with K7M2 EVs increased the overall mortality during the five-week study period in mice with K7M2 tumors↗
▶Ep 3 · 4:18
clinicalPre-education with K7M2 EVs increased the overall mortality during the five-week study period in mice with K7M2 tumors↗
▶Ep 3 · 4:44
clinicalK7M2 EV education led to increased metastatic burden in mice with K12 tumors↗
▶Ep 3 · 4:44
clinicalK7M2 EV education led to increased metastatic burden in mice with K12 tumors↗
▶Ep 3 · 5:01
clinicalPre-education with highly metastatic cell line EVs increases the metastasis seen in a normally low metastatic tumor↗
▶Ep 3 · 5:01
clinicalPre-education with highly metastatic cell line EVs increases the metastasis seen in a normally low metastatic tumor↗
▶Ep 3 · 5:09
clinicalNo mice with K12 tumors died in the study period↗
▶Ep 3 · 5:09
clinicalNo mice with K12 tumors died in the study period↗
▶Ep 3 · 5:14
clinicalExtracellular vesicles delivered prior to tumor implantation from highly metastatic K7M2 increase the metastatic burden of K7M2 tumors↗
▶Ep 3 · 5:14
clinicalExtracellular vesicles delivered prior to tumor implantation from highly metastatic K7M2 increase the metastatic burden of K7M2 tumors↗
▶Ep 3 · 5:24
clinicalIncreased metastatic burden from extracellular vesicles decreases overall survival↗
▶Ep 3 · 5:24
clinicalIncreased metastatic burden from extracellular vesicles decreases overall survival↗
▶Ep 3 · 5:30
clinicalExtracellular vesicles from highly metastatic K7M2 increase the metastatic burden of less metastatic K12 tumors↗
▶Ep 3 · 5:30
clinicalExtracellular vesicles from highly metastatic K7M2 increase the metastatic burden of less metastatic K12 tumors↗
▶Ep 3 · 5:39
quoteExtracellular vesicles can cause metastatic spread and therefore be a therapeutic target for osteosarcoma metastasis.↗
▶Ep 3 · 5:39
quoteExtracellular vesicles can cause metastatic spread and therefore be a therapeutic target for osteosarcoma metastasis.↗
▶Ep 3 · 6:27
clinicalAll cells in the body release EVs, so we can't just generally target EVs and hope to decrease the establishment of a pre-metastatic niche↗
▶Ep 3 · 6:27
clinicalAll cells in the body release EVs, so we can't just generally target EVs and hope to decrease the establishment of a pre-metastatic niche↗
▶Ep 3 · 6:48
clinicalIRF5 is a molecule being explored as a potential immunotherapy target in osteosarcoma EVs↗
▶Ep 3 · 6:48
clinicalIRF5 is a molecule being explored as a potential immunotherapy target in osteosarcoma EVs↗
▶Ep 3 · 7:10
quoteI don't think that it could treat the primary tumor as well as surgically removing it, which is, um, the current standard of care.↗
▶Ep 3 · 7:10
guidelineSurgically removing the primary tumor is the current standard of care for osteosarcoma↗
▶Ep 3 · 7:10
guidelineSurgically removing the primary tumor is the current standard of care for osteosarcoma↗
▶Ep 3 · 7:10
quoteI don't think that it could treat the primary tumor as well as surgically removing it, which is, um, the current standard of care.↗
▶Ep 3 · 7:31
clinicalThere are certain membrane EV markers, but they're shared by all types of EVs↗
▶Ep 3 · 7:31
clinicalThere are certain membrane EV markers, but they're shared by all types of EVs↗
▶Ep 3 · 7:40
clinicalThe differences in EVs by cell type have more to do with the contents inside of them than the targets on them, which makes them difficult to target as far as treatment↗
▶Ep 3 · 7:40
clinicalThe differences in EVs by cell type have more to do with the contents inside of them than the targets on them, which makes them difficult to target as far as treatment↗
▶Ep 3 · 7:57
clinicalStudies in dogs have shown ability to take serum of dogs with osteosarcoma and determine their likelihood of metastasis based on the EVs↗
▶Ep 3 · 7:57
clinicalStudies in dogs have shown ability to take serum of dogs with osteosarcoma and determine their likelihood of metastasis based on the EVs↗
Bailey Roberts, MD - Best of the Best in Pediatric Surgery 2024
▶Ep 20 · 1:04
quoteOsteosarcoma is a malignant pediatric tumor of the bone.↗
▶Ep 20 · 1:04
quoteOsteosarcoma is a malignant pediatric tumor of the bone.↗
▶Ep 20 · 1:08
epidemiologicalOsteosarcoma most commonly affects adolescents and young adults↗
▶Ep 20 · 1:08
epidemiologicalOsteosarcoma most commonly affects adolescents and young adults↗
▶Ep 20 · 1:11
clinicalThe most common site of metastasis in osteosarcoma is in the lung↗
▶Ep 20 · 1:11
epidemiologicalGross metastasis at the time of diagnosis is present about 20% of the time in osteosarcoma↗
▶Ep 20 · 1:11
clinicalThe most common site of metastasis in osteosarcoma is in the lung↗
▶Ep 20 · 1:11
epidemiologicalGross metastasis at the time of diagnosis is present about 20% of the time in osteosarcoma↗
▶Ep 20 · 1:19
epidemiologicalOverall survival in osteosarcoma worsens from 80% to about 20% for metastatic disease↗
▶Ep 20 · 1:19
epidemiologicalOverall survival in osteosarcoma worsens from 80% to about 20% for metastatic disease↗
▶Ep 20 · 1:25
clinicalRecurrence is very common in osteosarcoma, and the most common site of recurrence is in the lungs↗
▶Ep 20 · 1:25
clinicalRecurrence is very common in osteosarcoma, and the most common site of recurrence is in the lungs↗
▶Ep 20 · 1:32
quoteExtracellular vesicles or EVs are small nanoparticles released from all cells.↗
▶Ep 20 · 1:32
quoteExtracellular vesicles or EVs are small nanoparticles released from all cells.↗
▶Ep 20 · 1:38
clinicalExtracellular vesicles are carriers of amino acids, proteins, mRNA or other forms of cellular communication↗
▶Ep 20 · 1:38
clinicalExtracellular vesicles are carriers of amino acids, proteins, mRNA or other forms of cellular communication↗
▶Ep 20 · 1:45
clinicalEV content is unique and specific to the cells that released it, and therefore can be traced back to the cell type↗
▶Ep 20 · 1:45
clinicalEV content is unique and specific to the cells that released it, and therefore can be traced back to the cell type↗
▶Ep 20 · 1:52
clinicalEVs are involved in the metastatic cascade and implicated as potential markers for disease diagnosis and staging↗
▶Ep 20 · 1:52
clinicalEVs are involved in the metastatic cascade and implicated as potential markers for disease diagnosis and staging↗
▶Ep 20 · 1:59
clinicalEVs have been shown to change fibroblasts, inhibit T cell activity, and are involved in establishing a pre-metastatic niche↗
▶Ep 20 · 1:59
clinicalEVs have been shown to change fibroblasts, inhibit T cell activity, and are involved in establishing a pre-metastatic niche↗
▶Ep 20 · 2:14
quoteThe aim of our study was to determine if extracellular vesicles from osteosarcoma can establish a pre-metastatic niche and increase pulmonary metastasis in a mirroring model of osteosarcoma.↗
▶Ep 20 · 2:14
quoteThe aim of our study was to determine if extracellular vesicles from osteosarcoma can establish a pre-metastatic niche and increase pulmonary metastasis in a mirroring model of osteosarcoma.↗
▶Ep 20 · 2:31
clinicalK12 is a spontaneous osteosarcoma with low metastatic capability↗
▶Ep 20 · 2:31
clinicalK12 is a spontaneous osteosarcoma with low metastatic capability↗
▶Ep 20 · 2:35
clinicalK7M2 is a highly metastatic osteosarcoma derived from a spontaneous osteosarcoma that went through two reiterations of harvesting spontaneous lung metastases and reimplanting them into the mouse↗
▶Ep 20 · 2:35
clinicalK7M2 is a highly metastatic osteosarcoma derived from a spontaneous osteosarcoma that went through two reiterations of harvesting spontaneous lung metastases and reimplanting them into the mouse↗
▶Ep 20 · 2:57
clinicalThe K7M2 cell line is more aggressive and causes death at a much higher rate than K12↗
▶Ep 20 · 2:57
clinicalThe K7M2 cell line is more aggressive and causes death at a much higher rate than K12↗
▶Ep 20 · 3:59
clinicalPre-education with K7M2 EVs drastically increases the metastatic burden in the lungs of mice with K7M2 tumors↗
▶Ep 20 · 3:59
clinicalPre-education with K7M2 EVs drastically increases the metastatic burden in the lungs of mice with K7M2 tumors↗
▶Ep 20 · 4:18
clinicalPre-education with K7M2 EVs increased the overall mortality during the five-week study period in mice with K7M2 tumors↗
▶Ep 20 · 4:18
clinicalPre-education with K7M2 EVs increased the overall mortality during the five-week study period in mice with K7M2 tumors↗
▶Ep 20 · 4:44
clinicalK7M2 EV education led to increased metastatic burden in mice with K12 tumors↗
▶Ep 20 · 4:44
clinicalK7M2 EV education led to increased metastatic burden in mice with K12 tumors↗
▶Ep 20 · 5:01
clinicalPre-education with highly metastatic cell line EVs increases the metastasis seen in a normally low metastatic tumor↗
▶Ep 20 · 5:01
clinicalPre-education with highly metastatic cell line EVs increases the metastasis seen in a normally low metastatic tumor↗
▶Ep 20 · 5:09
clinicalNo mice with K12 tumors died in the study period↗
▶Ep 20 · 5:09
clinicalNo mice with K12 tumors died in the study period↗
▶Ep 20 · 5:14
clinicalExtracellular vesicles delivered prior to tumor implantation from highly metastatic K7M2 increase the metastatic burden of K7M2 tumors↗
▶Ep 20 · 5:14
clinicalExtracellular vesicles delivered prior to tumor implantation from highly metastatic K7M2 increase the metastatic burden of K7M2 tumors↗
▶Ep 20 · 5:24
clinicalIncreased metastatic burden from extracellular vesicles decreases overall survival↗
▶Ep 20 · 5:24
clinicalIncreased metastatic burden from extracellular vesicles decreases overall survival↗
▶Ep 20 · 5:30
clinicalExtracellular vesicles from highly metastatic K7M2 increase the metastatic burden of less metastatic K12 tumors↗
▶Ep 20 · 5:30
clinicalExtracellular vesicles from highly metastatic K7M2 increase the metastatic burden of less metastatic K12 tumors↗
▶Ep 20 · 5:39
quoteExtracellular vesicles can cause metastatic spread and therefore be a therapeutic target for osteosarcoma metastasis.↗
▶Ep 20 · 5:39
quoteExtracellular vesicles can cause metastatic spread and therefore be a therapeutic target for osteosarcoma metastasis.↗
▶Ep 20 · 6:27
clinicalAll cells in the body release EVs, so we can't just generally target EVs and hope to decrease the establishment of a pre-metastatic niche↗
▶Ep 20 · 6:27
clinicalAll cells in the body release EVs, so we can't just generally target EVs and hope to decrease the establishment of a pre-metastatic niche↗
▶Ep 20 · 6:48
clinicalIRF5 is a molecule being explored as a potential immunotherapy target in osteosarcoma EVs↗
▶Ep 20 · 6:48
clinicalIRF5 is a molecule being explored as a potential immunotherapy target in osteosarcoma EVs↗
▶Ep 20 · 7:10
guidelineSurgically removing the primary tumor is the current standard of care for osteosarcoma↗
▶Ep 20 · 7:10
quoteI don't think that it could treat the primary tumor as well as surgically removing it, which is, um, the current standard of care.↗
▶Ep 20 · 7:10
quoteI don't think that it could treat the primary tumor as well as surgically removing it, which is, um, the current standard of care.↗
▶Ep 20 · 7:10
guidelineSurgically removing the primary tumor is the current standard of care for osteosarcoma↗
▶Ep 20 · 7:31
clinicalThere are certain membrane EV markers, but they're shared by all types of EVs↗
▶Ep 20 · 7:31
clinicalThere are certain membrane EV markers, but they're shared by all types of EVs↗
▶Ep 20 · 7:40
clinicalThe differences in EVs by cell type have more to do with the contents inside of them than the targets on them, which makes them difficult to target as far as treatment↗
▶Ep 20 · 7:40
clinicalThe differences in EVs by cell type have more to do with the contents inside of them than the targets on them, which makes them difficult to target as far as treatment↗
▶Ep 20 · 7:57
clinicalStudies in dogs have shown ability to take serum of dogs with osteosarcoma and determine their likelihood of metastasis based on the EVs↗
▶Ep 20 · 7:57
clinicalStudies in dogs have shown ability to take serum of dogs with osteosarcoma and determine their likelihood of metastasis based on the EVs↗
