The effect of pre- and post-remote ischemic conditioning reduces the injury associated with intestinal ischemia/reperfusion
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In brief
In brief
Experimental rat study demonstrates that remote ischemic conditioning—brief cycles of limb ischemia—significantly reduces intestinal damage, inflammation, and oxidative stress caused by mesenteric artery occlusion. This protective effect occurred whether RIC was applied during ischemia or after reperfusion, suggesting potential clinical application in pediatric midgut volvulus cases.
Written by the GCMD Library team from the article.
Abstract
Purpose
Midgut volvulus is associated with intestinal ischemia/reperfusion (IR) injury and can progress to severe intestinal damage. Remote ischemic conditioning (RIC) reduces IR-induced injury in distant organs. The aim of this study was to investigate whether RIC protects the intestine from IR injury.
Methods
We investigated intestinal IR injury in 3 weeks old SD rats. Animals underwent: (i) sham laparotomy, (ii) intestinal IR injury, (iii) intestinal IR + RIC during ischemia, or (iv) intestinal IR + RIC after reperfusion. Intestinal IR injury was achieved by 45 min occlusion of superior mesenteric artery followed by de-occlusion. RIC was administered via four cycles of 5 min of hind limb ischemia followed by 5 min reperfusion. Animals were sacrificed 24 h after surgery and the ileum was harvested for evaluation.
Results
Intestinal injury was present after IR. However, this injury was reduced in both IR + RIC groups. Expression of inflammatory cytokine IL6 was lower in IR + RIC groups compared to IR alone. Carbonyl protein was also significantly lower in IR + RIC compared to IR, indicating lower oxidative stress in both IR + RIC groups.
Conclusion
Remote ischemic conditioning attenuated intestinal injury, inflammation, and oxidative stress in experimental intestinal ischemia/reperfusion injury. Remote ischemic conditioning may be useful in children with midgut volvulus to reduce the intestinal injury.
Level of evidence
Experimental study.
Type of study
Animal experiment.