Article1 min read·Published Jan 2022Older

Suspension culture promotes serosal mesothelial development in human intestinal organoids

Part of Intestinal Organoid Science & Bioengineering 49 items

pubmed.ncbi.nlm.nih.gov

Michael Helmrath

Pluripotent-stem-cell-derived human intestinal organoids (HIOs) model some aspects of intestinal development and disease, but current culture methods do not fully recapitulate the diverse cell types and complex organization of the human intestine and are reliant on 3D extracellular matrix or hydrogel systems, which limit experimental control and translational potential for regenerative medicine. We describe suspension culture as a simple, low-maintenance method for culturing HIOs and for promoting in vitro differentiation of an organized serosal mesothelial layer that is similar to primary human intestinal serosal mesothelium based on single-cell RNA sequencing and histological analysis. Functionally, HIO serosal mesothelium has the capacity to differentiate into smooth-muscle-like cells and exhibits fibrinolytic activity. An inhibitor screen identifies Hedgehog and WNT signaling as regulators of human serosal mesothelial differentiation. Collectively, suspension HIOs represent a three-dimensional model to study the human serosal mesothelium.

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Article · Jan 2022 · 1 min read

In brief

This study introduces suspension culture as a simplified method for growing human intestinal organoids that promotes development of an organized serosal mesothelial layer, closely mimicking native human intestinal tissue. The technique eliminates reliance on complex hydrogel systems and identifies Hedgehog and WNT signaling pathways as key regulators of mesothelial differentiation, advancing translational potential for regenerative medicine applications.

Written by the GCMD Library team from the article.

Michael Helmrath

Statements in this article

Current culture methods for pluripotent-stem-cell-derived human intestinal organoids do not fully recapitulate the diverse cell types and complex organization of the human intestine.
Established
Current human intestinal organoid culture methods are reliant on 3D extracellular matrix or hydrogel systems, which limit experimental control and translational potential for regenerative medicine.
Established
Suspension culture is a simple, low-maintenance method for culturing human intestinal organoids.
Research
Suspension culture promotes in vitro differentiation of an organized serosal mesothelial layer in human intestinal organoids that is similar to primary human intestinal serosal mesothelium based on single-cell RNA sequencing and histological analysis.
Research
Human intestinal organoid serosal mesothelium has the capacity to differentiate into smooth-muscle-like cells.
Research
Human intestinal organoid serosal mesothelium exhibits fibrinolytic activity.
Research
Hedgehog signaling regulates human serosal mesothelial differentiation in suspension-cultured intestinal organoids.
Research
WNT signaling regulates human serosal mesothelial differentiation in suspension-cultured intestinal organoids.
Research