Splenic development and injury in premature lambs supported by the artificial placenta
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In brief
In brief
Preclinical study evaluating splenic injury and development in premature lambs supported by artificial placenta technology for 10-14 days. Histologic analysis assessed inflammation, extramedullary hematopoiesis, and macrophage responses compared to gestational-age controls.
Written by the GCMD Library team from the article.
Introduction
The purpose of this study is to evaluate splenic effects during artificial placenta (AP) support.
Methods
AP lambs (118–121 d, n = 14) were delivered and placed on the AP support for a goal of 10–14 days. Cannulation used right jugular drainage and umbilical vein reinfusion. Early (ETC; 115–120 d; n = 7) and late (LTC; 125–131 d; n = 7) tissue controls were delivered and immediately sacrificed. Spleens were formalin fixed, H&E stained, and graded for injury, response to inflammation, and extramedullary hematopoiesis (EMH). CD68 and CD163 stains were used to assess for macrophage activation and density. Clinical variables were correlated with splenic scores. Groups were compared using Fisher's Exact Test and descriptive statistics. p