Routing Pathway of Syngeneic Donor Hematopoietic Stem Cells after Simple Intra-Amniotic Delivery
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In brief
In brief
Experimental study tracking luciferase-labeled syngeneic hematopoietic stem cells after intra-amniotic injection in rat fetuses to map cellular trafficking pathways. Research identifies HSC presence in fetal membranes, placenta, and amniotic fluid across multiple gestational timepoints, advancing understanding of prenatal stem cell delivery mechanisms.
Written by the GCMD Library team from the article.
Background
We sought to determine the pathway through which syngeneic hematopoietic stem cells (HSCs) delivered into the amniotic fluid can reach the fetal circulation.
Methods
Lewis rat fetuses were divided in two groups based on the content of intra-amniotic injections performed on gestational day 17 (E17; term=E21-22): either a suspension of luciferase-labeled syngeneic HSCs (n=137), or acellular luciferase (n=44). Samples from placenta, chorion, amnion, amniotic fluid, umbilical cord, and 8 fetal sites were procured at 5 daily time points thereafter until term for analysis.
Results
When controlled by acellular luciferase, donor HSCs were identified in the amnion, chorion, placenta, and amniotic fluid of fetuses receiving cells at all time points (p=0.033 to