Routing Kinetics of Human Immunoglobulin-G after Transamniotic Fetal Immunotherapy (TRAFIT) in a Rodent Model
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Read the article on jpedsurg.org ↗Article · Feb 2022 · 1 min read
In brief
In brief
Experimental study investigating the absorption pathways of human IgG after intra-amniotic injection in a rat model, tracking distribution across fetal tissues and fluids over three gestational timepoints. Research aims to elucidate mechanisms underlying transamniotic fetal immunotherapy, a minimally invasive approach for prenatal immunoglobulin delivery.
Written by the GCMD Library team from the article.
Purpose
The transamniotic route was recently discovered as a minimally invasive means of fetal immunoglobulin administration, however by unclear mechanisms. We sought to examine IgG routing after intra-amniotic delivery.
Methods
Sprague-Dawley fetuses (n=78) received intra-amniotic injections of 15mg/mL of human IgG on gestational-day 18 (E18; term=21-22 days). Amniotic fluid, amnion, chorion, placenta, fetal serum, liver, and stomach-aspirate samples were procured on E19, E20, and E21 for IgG quantification by ELISA. Statistical analysis was by median regression with Bonferroni-adjusted significance at p