Protective effects of lactoferrin on injured intestinal epithelial cells
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Read the article on jpedsurg.org ↗Article · Oct 2019 · 1 min read
In brief
In brief
This in vitro study demonstrates that lactoferrin protects rat and human intestinal epithelial cells from hydrogen peroxide-induced injury by modulating inflammatory markers (IL-6), intestinal stem cell markers (Lgr5), and proliferation pathways (Wnt/β-catenin), providing mechanistic insight into lactoferrin's protective role in necrotizing enterocolitis.
Written by the GCMD Library team from the article.
Purpose
Lactoferrin has been used as a milk supplement to prevent disease progression in necrotizing enterocolitis. However, the underlying mechanism is still unclear. We hypothesize that lactoferrin administration can modulate intestinal epithelial cell injury.
Methods
We established an in vitro model of epithelial cell injury by treating rat intestinal epithelial cells IEC-18 and human Caco-2 cells with hydrogen peroxide (H2O2), while lactoferrin was added as treatment at the same time. Live/dead cells were detected by immunofluorescence. Gene expression of inflammatory markers interleukin-6 (IL-6), intestinal stem cells (Lgr5), and proliferation marker (Wnt/β-catenin) were measured. Data was presented as mean ± SEM and compared using one-way ANOVA. A p-value