Primary exploring the value of metagenomic next‐generation sequencing in detecting pathogenic bacteria of cholangitis with biliary atresia after Kasai operation
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In brief
In brief
Study compares metagenomic next-generation sequencing (mNGS) to traditional blood culture for detecting cholangitis pathogens in 25 biliary atresia patients post-Kasai operation. mNGS demonstrated superior detection rate (73.3% vs 23.3%), faster turnaround time (16 vs 120 hours), and identified broader bacterial spectrum, supporting its use for targeted antibiotic therapy in this high-risk population.
Written by the GCMD Library team from the article.
Abstract
Purpose
To evaluate the value of metagenomic next‐generation sequencing (mNGS) in detecting pathogenic bacteria of cholangitis for patients with biliary atresia after Kasai operation.
Methods
This study retrospectively analyzed patients of biliary atresia with cholangitis after Kasai operation who were admitted to Xi’an Children’s Hospital from July 2019 to December 2021. Both blood culture and mNGS were carried out in all of these patients. We compared the detection rate of pathogenic bacteria, pathogenic bacteria spectrum, test time, inflammatory indicators and liver function. All the patients were followed up for 0.5–3 years to evaluate the onset of cholangitis and the survival status of autologous liver.
Results
This study included total of 30 cholangitis occurred in 25 patients. There were significant differences in the detection rate of pathogenic bacteria [23.3 vs.73.3%, P < 0.05] and the test time [120 (114.5–120) vs.16 (16–21) h, P < 0.001] between the blood culture and mNGS. These two methods showed significant statistical differences in comparing inflammatory indicators (CRP, PCT) and liver function (TB, DB, GGT) before and after anti-infection. Four kinds of bacteria were detected by blood cultures and ten kinds of bacteria were detected by mNGS. Cholangitis occurred 3 times in one case (4%) and twice in three cases (12%). Autologous liver survived in 17 cases (68%).
Conclusion
Comparing with traditional blood culture, mNGS is more efficient, convenient and accurate in the detection of pathogens. It provides a new method for accurately detecting pathogenic bacteria of cholangitis after Kasai operation.