Inhibiting hydrogen sulfide production in umbilical stem cells reduces their protective effects during experimental necrotizing enterocolitis
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In brief
In brief
Experimental study demonstrates that umbilical mesenchymal stem cells reduce necrotizing enterocolitis severity in neonatal mice through hydrogen sulfide production. When H2S synthesis was inhibited via siRNA, the protective effects were significantly diminished, confirming the critical role of paracrine H2S signaling in stem cell-mediated intestinal protection.
Written by the GCMD Library team from the article.
Introduction
Umbilical mesenchymal stem cells (USC) have been shown to reduce illness in animal models of necrotizing enterocolitis (NEC), possibly through the paracrine release of hydrogen sulfide (H2S). We hypothesized that animals treated with USCs with inhibited H2S synthesis would exhibit more severe disease.
Methods
NEC was induced in five-day-old mouse pups by formula feeding and hypoxic and hypothermic stress. Experimental groups received intraperitoneal injection of either saline vehicle or 80,000cells/gram of one of the following cell types: USC, USCs with negative-control siRNA, or USCs with targeted siRNA inhibition of the H2S-producing enzymes. Pups were monitored by clinical assessment and after euthanasia, intestine and lung histologic injury were scored. Tissue was homogenized, and concentrations of IL-6, IL-10, and VEGF were determined by ELISA. For statistical analysis, p