Increased protease activated receptors in the colon of patients with Hirschsprung's disease
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Read the article on jpedsurg.org ↗Article · Dec 2019 · 1 min read
In brief
In brief
Research study examining protease-activated receptor (PAR-1 and PAR-2) expression in Hirschsprung's disease patients reveals significantly elevated levels in both ganglionic and aganglionic bowel segments compared to controls. Findings suggest PAR dysregulation may contribute to Hirschsprung's-associated enterocolitis through effects on intestinal barrier function and motility.
Written by the GCMD Library team from the article.
Purpose
The pathophysiology of Hirschsprung's associated enterocolitis (HAEC) is not understood. Abnormal intestinal motility and altered intestinal epithelial barrier function have been suggested to play a key role in the causation of HAEC. Protease-activated receptors (PARs) 1 and 2, have been implicated in inflammatory reactions, intestinal permeability and modulation of motility in the gut.
Methods
We investigated PAR-1 and PAR-2 protein expression in aganglionic and ganglionic regions of patients with Hirschsprung's Disease (HSCR) (n = 10) versus normal control colon (n = 10). Protein distribution was assessed by using immunofluorescence and confocal microscopy. Gene and protein expression were quantified using quantitative real-time polymerase chain reaction (qPCR), western blot analysis, and densitometry.
Results
qPCR and Western blot analysis revealed that PAR-1 and PAR-2 expression was significantly increased in ganglionic and aganglionic bowel in HSCR compared to controls (p