Gonocyte transformation in congenital undescended testes: what is the role of inhibin-B in cell death?
link.springer.com shows its articles on its own site.
Read the article on link.springer.com ↗Article · Aug 2019 · 1 min read
In brief
In brief
Study examining whether inhibin-B levels influence gonocyte cell death in infants with undescended testes during minipuberty. Testicular biopsies from 10 boys undergoing orchidopexy showed no significant difference in apoptosis markers between normal and low inhibin-B groups, suggesting inhibin-B does not regulate gonocyte transformation.
Written by the GCMD Library team from the article.
Abstract
Purpose
Undescended testes (UDT) are subjected to heat stress, which can disturb gonocyte transformation as well as apoptosis. This study aims to describe the apoptosis pathway occurring during minipuberty of children with unilateral (UDT), and to investigate the role of inhibin-B.
Methods
Testicular biopsies at unilateral orchidopexy of 10 boys (6–9 months old) with normal inhibin-B (n = 5) or low inhibin-B (n = 5) were selected for immunohistochemistry and TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labelling) assay. Testicular tubules were labelled with antibodies against Anti-Müllerian hormone (AMH, Sertoli cell marker), mouse Vasa Homolog (MVH) and placental alkaline phosphatase (PLAP) (both germ cell markers), cleaved caspase3 (apoptotic marker), and followed by confocal imaging and cell counting with Fiji/ImageJ. Data were analyzed with GraphPad Prism.
Results
In males with low and normal inhibin-B, there was no statistical difference (p > 0.05) in the percentage of testicular tubules containing TUNEL + cells, number of cleaved caspase3 ± germ cells/tubule, total number of germ cells/tubule, and the percentage of fibrotic tubules or number of Sertoli cells/tubule.
Conclusions
These results suggest that inhibin-B does not regulate cell death of gonocytes and further studies are required to uncover any role of inhibin-B in gonocyte transformation.