Alterations of gut microbiota and serum bile acids are associated with parenteral nutrition-associated liver disease
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In brief
In brief
Study examining gut microbiota changes in a rat model of parenteral nutrition-associated liver disease (PNALD). Findings reveal altered bacterial composition with increased secondary bile acid biosynthesis pathways and enzymes, resulting in elevated secondary-to-primary bile acid ratios that may contribute to PNALD pathogenesis.
Written by the GCMD Library team from the article.
Abstract
Background
Parenteral nutrition-associated liver disease (PNALD) is a major complication of long-term parenteral nutrition (PN). The pathogenesis of PNALD remains unclear. We investigated the changes in taxonomic and functional composition of gut microbiota and serum bile acid levels in a rat model of PNALD.
Methods
Male 4-week-old Sprague Dawley rats received either total parenteral nutrition or standard chow with 0.9% saline for 7 days. The taxonomic composition of cecal microbiota and its functional composition associated with bile acid metabolism were measured.
Results
There were differences in taxonomic composition between the two groups. The abundance of the secondary bile acid biosynthesis pathway was higher in the TPN group (p < 0.05) with an increase in the percentage of bacteria expressing 7-alpha-hydroxysteroid dehydrogenase (p < 0.05). The abundance of enzymes associated with bile salt hydrolase was also higher (p < 0.05) in the TPN group. The TPN group showed a distinct bile acid profile characterized by a higher ratio of secondary bile acids to primary bile acids.
Conclusions
The alteration of bile acid-associated microbiota may lead to increased secondary bile acid production in a rat model of PNALD.