Adaptation of extracellular matrix to massive small bowel resection in mice
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Read the article on jpedsurg.org ↗Article · Mar 2020 · 1 min read
In brief
In brief
Proteomic analysis of murine ileal tissue 7 days post-50% proximal small bowel resection reveals significant extracellular matrix remodeling with 95 differentially regulated proteins. Study integrates mass spectrometry and transcriptional data to characterize ECM changes supporting adaptive cellular processes after massive bowel resection.
Written by the GCMD Library team from the article.
Background
Extracellular matrix (ECM) affects cell behavior, and vice versa. How ECM changes after small bowel resection (SBR) to support adaptive cellular processes has not been described. Here we characterize changes in ECM following SBR and integrate this with concomitant transcriptional perturbations.
Methods
A 50% proximal SBR or sham surgery was performed on mice. On postoperative day 7, ileal tissue was sequentially depleted of protein components to generate an ECM-enriched fraction. ECM was analyzed for protein composition using mass spectrometry with subsequent Ingenuity Pathway Analysis (IPA) to identify predicted pathways and upstream regulators. qPCR and RNA-sequencing (RNA-Seq) were performed to corroborate these predicted pathways.
Results
3034 proteins were differentially regulated between sham and SBR, of which 95 were significant (P