Article1 min read·Published Mar 2012Older

A nomenclature for intestinal in vitro cultures

Part of Intestinal Stem Cells & Regeneration 24 items

pubmed.ncbi.nlm.nih.gov

Michael Helmrath

Many advances have been reported in the long-term culture of intestinal mucosal cells in recent years. A significant number of publications have described new culture media, cell formations, and growth patterns. Furthermore, it is now possible to study, e.g., the capabilities of isolated stem cells or the interactions between stem cells and mesenchyme. However, at the moment there is significant variation in the way these structures are described and named. A standardized nomenclature would benefit the ability to communicate and compare findings from different laboratories using the different culture systems. To address this issue, members of the NIH Intestinal Stem Cell Consortium herein propose a systematic nomenclature for in vitro cultures of the small and large intestine. We begin by describing the structures that are generated by preparative steps. We then define and describe structures produced in vitro, specifically: enterosphere, enteroid, reconstituted intestinal organoid, induced intestinal organoid, colonosphere, colonoid, and colonic organoid.

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Article · Mar 2012 · 1 min read

In brief

This consensus paper from the NIH Intestinal Stem Cell Consortium establishes standardized terminology for intestinal cell culture systems. The authors define key terms including enterosphere, enteroid, colonoid, and various organoid types to improve communication and comparison across research laboratories studying intestinal stem cells and tissue models.

Written by the GCMD Library team from the article.

Michael Helmrath

Statements in this article

A standardized nomenclature for intestinal in vitro cultures would benefit the ability to communicate and compare findings from different laboratories using different culture systems.
Established
Enterosphere refers to a three-dimensional sphere derived from small intestinal crypts or isolated stem cells grown in suspension culture.
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Enteroid refers to a three-dimensional structure derived from small intestinal crypts or isolated stem cells that contains a central lumen and demonstrates crypt-villus organization.
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Reconstituted intestinal organoid refers to a three-dimensional structure formed by recombining isolated intestinal epithelial cells with mesenchymal cells.
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Induced intestinal organoid refers to a three-dimensional intestinal structure derived from pluripotent stem cells through directed differentiation.
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Colonosphere refers to a three-dimensional sphere derived from colonic crypts or isolated colonic stem cells grown in suspension culture.
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Colonoid refers to a three-dimensional structure derived from colonic crypts or isolated colonic stem cells that contains a central lumen and demonstrates crypt organization.
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Colonic organoid refers to either a reconstituted colonic organoid formed by recombining colonic epithelial cells with mesenchymal cells, or an induced colonic organoid derived from pluripotent stem cells.
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