Characterization of the congenital diaphragmatic hernia model in C57BL/6J fetal mice: a step toward lineage tracing experiments

This study establishes a reproducible CDH model in C57BL/6J mice using nitrofen+bisdiamine, achieving 36% CDH incidence with characteristic lung hypoplasia, impaired growth, and reduced vascularization. The model successfully replicates key CDH pathophysiology, enabling future lineage tracing studies to investigate cellular fate during diaphragmatic and pulmonary development.