Label-free detection of human enteric nerve system using Raman spectroscopy: A pilot study for diagnosis of Hirschsprung disease

Pilot study demonstrates Raman spectroscopy can detect enteric nerve system presence in human intestinal tissue without labels or dyes, potentially enabling real-time intraoperative identification of ganglionic versus aganglionic bowel segments in Hirschsprung disease. Principal component analysis successfully discriminated ganglion from muscle layers in surgical specimens.