Article1 min read·Published Aug 2013Older

Impact of ileocecal resection and concomitant antibiotics on the microbiome of the murine jejunum and colon

Part of Short Bowel Syndrome & Intestinal Failure 8 items

pubmed.ncbi.nlm.nih.gov

Michael Helmrath

Ileocecal resection (ICR) is a commonly required surgical intervention in unmanageable Crohn's disease and necrotizing enterocolitis. However, the impact of ICR, and the concomitant doses of antibiotic routinely given with ICR, on the intestinal commensal microbiota has not been determined. In this study, wild-type C57BL6 mice were subjected to ICR and concomitant single intraperitoneal antibiotic injection. Intestinal lumen contents were collected from jejunum and colon at 7, 14, and 28 days after resection and compared to non-ICR controls. Samples were analyzed by 16S rRNA gene pyrosequencing and quantitative PCR. The intestinal microbiota was altered by 7 days after ICR and accompanying antibiotic treatment, with decreased diversity in the colon. Phylogenetic diversity (PD) decreased from 11.8 ± 1.8 in non-ICR controls to 5.9 ± 0.5 in 7-day post-ICR samples. There were also minor effects in the jejunum where PD values decreased from 8.3 ± 0.4 to 7.5 ± 1.4. PCoA analysis indicated that bacterial populations 28 days post-ICR differed significantly from non-ICR controls. Moreover, colon and jejunum bacterial populations were remarkably similar 28 days after resection, whereas the initial communities differed markedly. Firmicutes and Bacteroidetes were the predominant phyla in jejunum and colon before ICR; however, Firmicutes became the vastly predominant phylum in jejunum and colon 28 days after ICR. Although the microbiota returned towards a homeostatic state, with re-establ

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Article · Aug 2013 · 1 min read

In brief

This murine study demonstrates that ileocecal resection combined with perioperative antibiotics significantly disrupts intestinal microbiota, reducing colonic diversity by 50% at 7 days and causing persistent dysbiosis with Firmicutes predominance at 28 days. The findings reveal that jejunal and colonic bacterial communities converge post-resection, suggesting fundamental alterations in gut ecology that may inform postoperative management in Crohn's disease and NEC patients.

Written by the GCMD Library team from the article.

Michael Helmrath

Statements in this article

Ileocecal resection is a commonly required surgical intervention in unmanageable Crohn's disease and necrotizing enterocolitis.
Established
Phylogenetic diversity in the colon decreased from 11.8 ± 1.8 in non-ICR controls to 5.9 ± 0.5 at 7 days post-ICR with antibiotic treatment.
Research
Phylogenetic diversity in the jejunum decreased from 8.3 ± 0.4 to 7.5 ± 1.4 at 7 days post-ICR with antibiotic treatment.
Research
Bacterial populations at 28 days post-ICR differed significantly from non-ICR controls.
Research
Colon and jejunum bacterial populations were remarkably similar 28 days after resection, whereas the initial communities differed markedly.
Research
Firmicutes became the vastly predominant phylum in jejunum and colon 28 days after ICR, whereas Firmicutes and Bacteroidetes were the predominant phyla before ICR.
Research