Article1 min read·Published Jun 2018Older

Mechanically induced development and maturation of human intestinal organoids in vivo

Part of Intestinal Organoid Science & Bioengineering 49 items

pubmed.ncbi.nlm.nih.gov

Michael Helmrath

The natural ability of stem cells to self-organize into functional tissue has been harnessed for the production of functional human intestinal organoids. Although dynamic mechanical forces play a central role in intestinal development and morphogenesis, conventional methods for the generation of intestinal organoids have relied solely on biological factors. Here, we show that the incorporation of uniaxial strain, by using compressed nitinol springs, in human intestinal organoids transplanted into the mesentery of mice induces growth and maturation of the organoids. Assessment of morphometric parameters, transcriptome profiling, and functional assays of the strain-exposed tissue revealed higher similarities to native human intestine, with regards to tissue size and complexity, and muscle tone. Our findings suggest that the incorporation of physiologically relevant mechanical cues during the development of human intestinal tissue enhances its maturation and enterogenesis.

pubmed.ncbi.nlm.nih.gov shows its articles on its own site.

Read the article on pubmed.ncbi.nlm.nih.gov ↗

Article · Jun 2018 · 1 min read

In brief

This study demonstrates that applying mechanical strain via nitinol springs to transplanted human intestinal organoids significantly enhances their growth and maturation to more closely resemble native human intestine. The findings highlight the critical role of physiological mechanical forces in tissue development beyond traditional biological factor-based approaches.

Written by the GCMD Library team from the article.

Michael Helmrath

Statements in this article

Stem cells have the natural ability to self-organize into functional tissue, which has been harnessed for the production of functional human intestinal organoids.
Established
Dynamic mechanical forces play a central role in intestinal development and morphogenesis.
Established
Conventional methods for the generation of intestinal organoids have relied solely on biological factors.
Established
Incorporation of uniaxial strain using compressed nitinol springs in human intestinal organoids transplanted into the mesentery of mice induces growth and maturation of the organoids.
Research
Strain-exposed tissue showed higher similarities to native human intestine with regards to tissue size and complexity and muscle tone based on morphometric parameters, transcriptome profiling, and functional assays.
Research
Incorporation of physiologically relevant mechanical cues during the development of human intestinal tissue enhances its maturation and enterogenesis.
Research