Article1 min read·Published Jan 2023Older

In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice

Part of Intestinal Organoid Science & Bioengineering 49 items

pubmed.ncbi.nlm.nih.gov

Michael Helmrath

Human intestinal organoids (HIOs) derived from pluripotent stem cells provide a valuable model for investigating human intestinal organogenesis and physiology, but they lack the immune components required to fully recapitulate the complexity of human intestinal biology and diseases. To address this issue and to begin to decipher human intestinal-immune crosstalk during development, we generated HIOs containing immune cells by transplanting HIOs under the kidney capsule of mice with a humanized immune system. We found that human immune cells temporally migrate to the mucosa and form cellular aggregates that resemble human intestinal lymphoid follicles. Moreover, after microbial exposure, epithelial microfold cells are increased in number, leading to immune cell activation determined by the secretion of IgA antibodies in the HIO lumen. This in vivo HIO system with human immune cells provides a framework for future studies on infection- or allergen-driven intestinal diseases.

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Article · Jan 2023 · 1 min read

In brief

Researchers transplanted human intestinal organoids into humanized mice to create immune-competent tissue models. Human immune cells migrated to form lymphoid follicle-like structures, and microbial exposure triggered M cell development and IgA production, establishing a platform for studying intestinal immune interactions and disease mechanisms.

Written by the GCMD Library team from the article.

Michael Helmrath

Statements in this article

Human intestinal organoids derived from pluripotent stem cells lack the immune components required to fully recapitulate the complexity of human intestinal biology and diseases.
Established
Human intestinal organoids containing immune cells can be generated by transplanting HIOs under the kidney capsule of mice with a humanized immune system.
Research
Human immune cells temporally migrate to the mucosa and form cellular aggregates that resemble human intestinal lymphoid follicles in transplanted HIOs.
Research
After microbial exposure, epithelial microfold cells are increased in number in HIOs with immune cells.
Research
Microbial exposure leads to immune cell activation determined by the secretion of IgA antibodies in the HIO lumen.
Research
This in vivo HIO system with human immune cells provides a framework for future studies on infection- or allergen-driven intestinal diseases.
Research