Suture techniques and patch materials using an in-vitro model for watertight closure of in-utero spina bifida repair

In-vitro study evaluating surgical closure techniques for in-utero spina bifida repair using a chicken thigh model to simulate fetal tissue under physiologic CSF pressures. Findings demonstrate minimal wound edge traction optimizes primary closures while locking sutures provide superior watertight seal for patch-based repairs, offering technical guidance to reduce postoperative CSF leakage and Chiari II malformation rates.