Transamniotic stem cell therapy (TRASCET) in a rabbit model of spina bifida
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Read the article on jpedsurg.org ↗Article · Dec 2018 · 1 min read
In brief
In brief
Experimental study demonstrating that transamniotic injection of concentrated amniotic fluid mesenchymal stem cells can induce partial skin coverage of spina bifida defects in a rabbit model. This approach represents a potential minimally invasive prenatal treatment strategy that could complement or serve as an alternative to open fetal surgery for neural tube defects.
Written by the GCMD Library team from the article.
Purpose
Transamniotic stem cell therapy (TRASCET) with select mesenchymal stem cells (MSCs) has been shown to induce partial or complete skin coverage of spina bifida in rodents. Clinical translation of this emerging therapy hinges on its efficacy in larger animal models. We sought to study TRASCET in a model requiring intra-amniotic injections 60 times larger than those performed in the rat.
Methods
Rabbit fetuses (n = 65) with surgically created spina bifida were divided into three groups. One group (untreated) had no further manipulations. Two groups received volume-matched intra-amniotic injections of either saline or a concentrated suspension of amniotic fluid MSCs (afMSCs) at the time of operation. Infused afMSCs consisted of banked heterologous rabbit afMSCs with mesenchymal identity confirmed by flow cytometry, labeled with green fluorescent protein. Defect coverage at term was blindly categorized only if the presence of a distinctive neoskin was confirmed histologically. Statistical comparisons were by logistic regression and the likelihood ratio test.
Results
Among survivors with spina bifida (n = 19), there were statistically significant higher rates of defect coverage (all partial) in the afMSC group when compared with the saline and untreated groups (0–50%; p = 0.022–0.036), with no difference between the saline and untreated groups (p = 1.00). Donor afMSCs were identified locally, though sparsely and not in the neoskin.
Conclusions
Concentrated intra-amniotic injection of amniotic mesenchymal stem cells can induce partial coverage of experimental spina bifida in a leporine model. Transamniotic stem cell therapy may become a feasible strategy in the prenatal management of spina bifida.
Level of Evidence
N/A (animal and laboratory study).