The effects of tracheal occlusion on Wnt signaling in a rabbit model of congenital diaphragmatic hernia
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In brief
In brief
Experimental study in fetal rabbits demonstrates that tracheal occlusion reverses pulmonary hypoplasia in CDH and examines the molecular mechanisms through Wnt signaling pathway analysis. Research validates the rabbit CDH model and investigates how TO intervention affects lung development at the genetic level.
Written by the GCMD Library team from the article.
Purpose
Tracheal occlusion (TO) reverses pulmonary hypoplasia (PH) in congenital diaphragmatic hernia (CDH), but its mechanism of action remains poorly understood. Wnt signaling plays a critical role in lung development, but few studies exist. The purpose of our study was to a) confirm that our CDH rabbit model produced PH which was reversed by TO and b) determine the effects of CDH +/− TO on Wnt signaling.
Methods
CDH was created in fetal rabbits at 23 days, TO at 28 days, and lung collection at 31 days. Lung body weight ratio (LBWR) and mean terminal bronchiole density (MTBD) were determined. mRNA and miRNA expression was determined in the left lower lobe using RT-qPCR.
Results
Fifteen CDH, 15 CDH + TO, 6 sham CDH, and 15 controls survived and were included in the study. LBWR was low in CDH, while CDH + TO was similar to controls (p = 0.003). MTBD was higher in CDH fetuses and restored to control levels in CDH + TO (p