Reduced expression of the NLRP6 inflammasome in the colon of patients with Hirschsprung’s disease
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In brief
In brief
Study demonstrates significantly reduced NLRP6 inflammasome expression in both aganglionic and ganglionic colon segments of Hirschsprung's disease patients compared to controls. Findings suggest NLRP6 dysregulation may contribute to altered intestinal immunity and microbiome disruption underlying Hirschsprung's-associated enterocolitis pathogenesis.
Written by the GCMD Library team from the article.
Purpose
Hirschsprung's associated enterocolitis (HAEC) is the most common cause of morbidity and mortality in Hirschsprung's Disease (HSCR). The pathogenesis of HAEC remains unsatisfactorily understood. Mounting evidence of an altered microbiome in patients with HSCR adds a new angle to the pathogenesis of HAEC. NLRP6 is a member of the nucleotide-binding domain, leucine-rich-repeat-containing (NLR) innate immune receptor family, a multiprotein complex that functions as a sensor of damage-associated molecular patterns. Known as inflammasomes they have been implicated in the regulation of colonic microbial ecology and by alteration, regulators of inflammation. We designed this study to test the hypothesis that NLRP6 expression is altered in the colon of patients with HSCR.
Methods
We investigated NLPR6 protein expression in both the aganglionic and ganglionic regions of HSCR patients (n = 10) versus healthy control colon (n = 10). Protein distribution was assessed by using immunofluorescence and confocal microscopy. Gene and protein expressions were quantified using quantitative real-time polymerase chain reaction (qPCR), Western blot analysis, and densitometry.
Main results
qPCR and Western blot analysis revealed that NLRP6 is expressed in the colon of patients with HSCR. NLRP6 expression was significantly decreased (p