Human skin-derived precursor cells xenografted in aganglionic bowel
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Read the article on jpedsurg.org ↗Article · Apr 2020 · 1 min read
In brief
In brief
Study demonstrates that human skin-derived precursor cells (hSKPs) transplanted into chemically denervated porcine colon survive and express neuroglial markers in myenteric and submucosal plexuses. Findings suggest hSKPs may offer a regenerative therapy approach for restoring enteric nervous system function in Hirschsprung disease patients.
Written by the GCMD Library team from the article.
Abstract
Purpose
One in 5000 newborns is diagnosed with Hirschsprung disease each year in the United States. The potential of employing neural crest stem cells to restore the enteric nervous system has been investigated. Skin-derived precursor cells (SKPs) are multipotent progenitor cells that can differentiate into neurons and gliocytes in vitro and generate enteric ganglion-like structures in rodents. Here we examined the behavior of human SKPs (hSKPs) after their transplantation into a large animal model of colonic aganglionosis.
Methods
Juvenile minipigs underwent a chemical denervation of the colon to establish an aganglionosis model. The hSKPs were generated from human foreskin and were cultured in neuroglial-selective medium. Cells were labeled with a fluorescent dye and were injected into the porcine aganglionic colon. After one week, transplanted hSKPs were assessed by immunofluorescence for markers of multipotency and neuroglial differentiation.
Results
In culture, hSKPs expressed nestin and S100b indicative of neuroglial precursors. After xenografting in pigs, hSKPs were identified in the myenteric and submucosal plexuses of the colons. The hSKPs expressed nestin and early neuroglial differentiation markers.
Conclusions
Human SKPs transplanted into aganglionic colon demonstrated immunophenotypes of neuroglial progenitors, suggesting their potential use for Hirschsprung disease.
Level of evidence