Establishment of a biobank for human lung tissues of congenital diaphragmatic hernia and congenital pulmonary airway malformation
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Read the article on jpedsurg.org ↗Article · May 2019 · 1 min read
In brief
In brief
Researchers established a biobank of lung tissue samples from 16 CDH and 18 CPAM patients with age-matched controls, spanning gestational ages and childhood. The archived formalin-fixed paraffin-embedded tissues showed good RNA quality and are linked to clinical data, enabling future translational studies of congenital lung disease pathogenesis.
Written by the GCMD Library team from the article.
Background
Human tissue samples are an invaluable and little available source of information for translational studies of congenital lung diseases such as Congenital Diaphragmatic Hernia (CDH) or Congenital Pulmonary Airway Malformation (CPAM).
Purpose
We aimed to establish a human lung tissue biobank of CDH and CPAM patients together with age-matched controls, coupled with a clinical database.
Methods
Pathology records from autopsies or surgical specimens for CDH and CPAM cases between 1980 and 2017 were reviewed. For surviving individuals, clinical patient data was obtained from corresponding pediatric surgery reports. Formalin-fixed, paraffin-embedded tissues of patients and age-matched controls were systematically stored for further translational studies. RNA integrity was determined on selected CDH blocks.
Results
A total of 16 CDH and 18 CPAM and age-matched control lung tissue blocks were included in our biobank. Ages ranged from 22 to 41 weeks of gestation (GA) in CDH (33.9 ± 6.35 weeks) and 26 weeks (GA) and 12 years in CPAM (2.3 ± 3.7 y). RNA isolation from CDH and control blocks yielded good RNA quality (OD 260/280 ratio: 2.01–2.09, OD 260/230 ratio: 2.04–2.09).
Conclusion
We established a unique human biobank for CDH and CPAM tissues. The combination with clinical patient data will allow us to design future translational studies to improve our understanding of the disease pathogenesis of these congenital malformations.