Dithizone-induced Paneth cell disruption significantly decreases intestinal perfusion in the murine small intestine
jpedsurg.org shows its articles on its own site.
Read the article on jpedsurg.org ↗Article · Mar 2019 · 1 min read
In brief
In brief
Experimental study in immature mice demonstrates that chemical disruption of Paneth cells with dithizone significantly reduces intestinal microvascular perfusion, associated with decreased nitric oxide synthase expression. Findings support the role of Paneth cells in regulating intestinal blood flow and provide mechanistic insight into necrotizing enterocolitis pathophysiology.
Written by the GCMD Library team from the article.
Purpose
Necrotizing enterocolitis is associated with decreased intestinal perfusion and ischemia. Paneth cells, specialized epithelial cells, have been shown to regulate the intestinal vasculature and disruption of these cells has been associated with NEC. We hypothesized that Paneth cell disruption in immature mice intestine would decrease the perfusion of the intestinal microvasculature.
Methods
Paneth cells were disrupted in P14–16 mice using chemical (dithizone) and transgenic (diphtheria toxin) methodology. Six hours after Paneth cell disruption, Dylight 488 was injected directly into the left ventricle and allowed to perfuse for 5 minutes prior to intestinal harvesting. Tissue samples were evaluated with confocal fluorescence microscopy to quantify intestinal perfusion and samples were quantified by real time RT-PCR for gene expression.
Results
Dithizone treatment significantly decreased intestinal perfusion compared to controls (p 0.21). Intestines from all treatment groups had similar PECAM staining, but intestines treated with dithizone had significantly decreased nNOS and iNOS gene expression compared to controls (p